original articleIssue 23 (3) 2024 pp. 337-344
Clara Espinoza Silva1, Judith De la Cruz2, Wiilfredo Mamani2, Omar Flores Ramos1, Edgar Rojas Zacarias1, Greta Hinostroza Quiñonez1
2Facultad de Ingenieria en Industrias Alimentarias, Universidad Nacional del Centro del Perú, El Tambo Huancayo, Perù
Cytotoxicity of saponin nanoparticles from quinoa (Chenopodium quinoa Willd.) in the MC-F7 cell line
Background. The effect of saponin nanoparticles from Chenopodium quinoa Willd. on MC-F7 (breast cancer) cell lines was evaluated. The ionic gelation method with chitosan and pentasodium tripolyphosphate was used to synthesize nanoparticles. Saponins were extracted from quinoa industry residues using ultrasound.
Materials and methods. The effect of pH (5, 6, 7) and pentasodium tripolyphosphate concentration (1, 2 and 3 mg/mL) was evaluated; the treatment with pH 6 and pentasodium tripolyphosphate concentration of 3 mg/mL obtained the best encapsulation efficiency. The nanoparticles had a Z potential of –32.9 V, a polydispersity of 0.282, and a particle size of 758.75 nm. The cytotoxicity of these nanoparticles formed in MC-F7 cells was tested and an IC50 value of 4.5 ug/mL was obtained.
Conclusion. The in vitro cytotoxic activity of Chenopodium quinoa Willd. saponin nanoparticles in MCF-7 cells was demonstrated using chitosan and pentasodium tripolyphosphate as a cross-linking material at a concentration of 3 mg/mL and pH 6, with an IC50 value of 4.5 mg/mL. These findings suggest its potential high bioactivity, which should be followed by further in vitro studies on cellular and molecular effects on cancer and normal cells.
Keywords: ionic gelation, saponin, nanoparticles, anticancer
https://www.food.actapol.net/volume23/issue3/6_3_2024.pdf
https://doi.org/10.17306/J.AFS.001236
MLA | Silva, Clara Espinoza, et al. "Cytotoxicity of saponin nanoparticles from quinoa (Chenopodium quinoa Willd.) in the MC-F7 cell line." Acta Sci.Pol. Technol. Aliment. 23.3 (2024): 337-344. https://doi.org/10.17306/J.AFS.001236 |
APA | Silva C. E., De la Cruz J., Mamani W., Ramos O. F., Zacarias E. R., Quiñonez G. H. (2024). Cytotoxicity of saponin nanoparticles from quinoa (Chenopodium quinoa Willd.) in the MC-F7 cell line. Acta Sci.Pol. Technol. Aliment. 23 (3), 337-344 https://doi.org/10.17306/J.AFS.001236 |
ISO 690 | SILVA, Clara Espinoza, et al. Cytotoxicity of saponin nanoparticles from quinoa (Chenopodium quinoa Willd.) in the MC-F7 cell line. Acta Sci.Pol. Technol. Aliment., 2024, 23.3: 337-344. https://doi.org/10.17306/J.AFS.001236 |