Acta Scientiarum Polonorum Technologia Alimentaria

Background. Selenium zinc chromium is an essential nutrient for humans and animals, and the free form of selenium zinc chromium state is not easily absorbed and is highly toxic. Yeast has characteristics such as easy growth and a high absorption rate of trace elements, which make it an ideal carrier for enrichment of trace elements. Therefore, in order to solve the problems of the low absorption rate of trace elements and the difficulty of effective daily supplementation, this study will examine the use of yeast to enrich more than two kinds of nutrients at the same time and to obtain and identify the strain with a good enrichment effect, high biomass and a broad development prospect.
Methods. Sixteen yeast strains were selected in order to study their enrichment capacity for selenium, zinc and chromium, respectively, using enrichment amount and conversion rate as screening indicators. The strains were then identified by sequence analysis of the 26S rDNA D1/D2 region.
Results. The strain with a better enrichment effect of selenium, zinc and chromium was obtained as Saccharomyces boulardii L2. When the compound nutrients sodium selenite (30 μg/mL), zinc sulfate (200 μg/mL) and chromium chloride (100 μg/mL) were added to the medium, the selenium content of the yeast was obtained, and the zinc content and chromium content were 917.37 μg/g, 1202.3 μg/g, 680.11 μg/g, respectively. The biomass was 19.58 g/L.
Conclusion. Fortified yeast enriched with selenium, zinc and chromium was obtained using S. boulardii L2 as a carrier. This study provides the theoretical basis and technical support for the preparation of yeast rich in selenium, zinc and chromium.