original articleIssue 21 (3) 2022 pp. 239-250
Anna Muzykiewicz-Szymańska, Anna Nowak, Katarzyna Florkowska, Adam Klimowicz
Optimization of ultrasound-assisted extraction of fresh and frozen Mirabelle plum to enhance antioxidant potential, polyphenols, plant pigments, and phenolic acid content
Background. The Mirabelle plum fruit is a known and valuable source of biologically active compounds, including antioxidants. This fruit is used in the food industry due to its savory taste as well. The leaves, despite their high content of active compounds, are used much less. This study aimed to optimize the extraction process of Mirabelle plum leaf and fruit. The effect of the freezing-thawing process on the antioxidant activity of extracts was evaluated, as was the content of selected phenolic compounds. The concentration of some plant pigments in fresh and frozen raw material and the content of a few phenolic acids in the selected leaf extracts were also determined.
Materials and methods. The extracts of Mirabelle plum leaf and fruit (peel and flesh separately) were prepared using ultrasound-assisted extraction. Evaluation of antioxidant activity was performed using in vitro techniques such as DPPH, ABTS, FIC, CUPRAC, and FRAP. The polyphenol, flavonoid, anthocyanin, chlorophyll, and carotenoid contents were also determined spectrophotometrically, as was the concentration of some phenolic acids in the selected leaf extracts by HPLC.
Results. The leaf extracts had higher antioxidant activity, polyphenols, and plant pigment contents than in the fruit. The lowest potential was observed for flesh extracts. The freezing-thawing process had rather an unfavorable effect on the activity of the leaf and peel extracts. The 120–180-minute extraction seemed to be more effective as compared to the 15–30-minute process. However, the prolongation of extraction up to 240 inutes led to a decrease in antioxidant potential and total polyphenol content. The leaf extracts had a relatively high concentration of phenolic acids, particularly chlorogenic acid (2.833 to 5.035 mg/100 mL of extract, on average).
Conclusion. To sum up, many factors, such as the plant part, extraction time, and the freezing-thawing process, could affect the antioxidant activity and content of biologically active agents in Mirabelle plum extracts. Due to the high content of active compounds including antioxidants in the Mirabelle leaves, their application in many areas of industry could be taken into consideration.
Keywords: antioxidant activity, freezing-thawing process, ultrasound-assisted extraction, plant pigments, polyphenols, Prunus domestica L.
https://www.food.actapol.net/volume21/issue3/1_3_2022.pdf
https://doi.org/10.17306/J.AFS.2022.0932
MLA | Muzykiewicz-Szymańska, Anna, et al. "Optimization of ultrasound-assisted extraction of fresh and frozen Mirabelle plum to enhance antioxidant potential, polyphenols, plant pigments, and phenolic acid content." Acta Sci.Pol. Technol. Aliment. 21.3 (2022): 239-250. https://doi.org/10.17306/J.AFS.2022.0932 |
APA | Muzykiewicz-Szymańska A., Nowak A., Florkowska K., Klimowicz A. (2022). Optimization of ultrasound-assisted extraction of fresh and frozen Mirabelle plum to enhance antioxidant potential, polyphenols, plant pigments, and phenolic acid content. Acta Sci.Pol. Technol. Aliment. 21 (3), 239-250 https://doi.org/10.17306/J.AFS.2022.0932 |
ISO 690 | MUZYKIEWICZ-SZYMAńSKA, Anna, et al. Optimization of ultrasound-assisted extraction of fresh and frozen Mirabelle plum to enhance antioxidant potential, polyphenols, plant pigments, and phenolic acid content. Acta Sci.Pol. Technol. Aliment., 2022, 21.3: 239-250. https://doi.org/10.17306/J.AFS.2022.0932 |